Agarose gel electrophoresis

bushra.x

New member
Apr 20, 2016
3
0
0
2
#1

My ladder is 100bp.

I have two primers to test out. Primer Y which amplifies up to a product of 200bp and primer Z which amplifies up to 150bp fragment.

I'm not very sure of how this all works. What does this mean? I thought that the ladder has to be bigger than the size of the primer. How can you measure with 100bp ladder.
 

gsovak

Administrator
Staff member
Nov 16, 2015
259
0
16
2
#2

Yes

You need to use a bigger ladder 1K will do it.



https://www.neb.com/products/n3232-1-kb-dna-ladder