help regarding fixation

AllieA

New member
Nov 16, 2015
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#1
Hi, I'm new to histology and am trying to fix my mice in order to preserve their diaphragm and heart in relaxed state. It seems that if I flush with KCL before formalin, the heart is relaxed, but the diaphragm contracts. If I flush with saline before formalin the diaphragm looks good but the heart is contracted.

Does any body else have a solution they like to use, or any tips at all?

Thanks!