Help with sample preparation in 0.01M HCL in the presence of EDTA and Sodium Metabisulfite

kellerro

New member
Oct 22, 2018
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#1
Hello,

I am trying to follow a protocol for an ELISA that measures catecholamines. The product says that in order to prevent catecholamine degradation you can add EDTA (final concentration 1mM) and sodium metabisulfite (final concentration 4mM) directly to the sample tissue. Does this mean that I need to homogenise the sample and determine the volume before I can know how much EDTA and sodium metabisulfite to add?

It also says that for sample preparation tissue can be homogenized in 0.01 N HCl in the presence of EDTA and sodium metabisulfite. How to I calculate the amount of HCl to add in order to homogenize my samples?


Here is the protocol: https://rmdiagnostics.com/content/Instructions/BA E-5600 en.pdf

Any help is appreciated!