Histology fixative (lugol's iodine fixation)

ebene29

New member
Nov 16, 2015
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#1
Hello,

My question will probably sound stupid for experts. I need to perform ELISA and LFIA on fixed phytoplankton. The common fixative is lugol's iodine solution for practical reasons. Is there any cautions to take ?

I've done few experiments. I lost the signal with "high" concentrations of lugol 's iodine (>1%).

I couldn't find any detailed paper about the fixation mechanisms. I am sure it has been described .

I would be glad for any advices or papers .

Thanks in advance for your help.