In vivo gene silencing in mice

Nov 16, 2015
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#1
At a meeting this summer, I heard a talk by the company Alnylam about in vivo RNAi in mice using siRNA complexed to liposomal nanoparticles.

I am now starting to see this technique show up in manuscripts. I went to the companies website but couldnt find the particles. Does anyone know where to get these particles? Also what is the efficiency? Are they first pass liver only or do they work throughout the body?

Here is a methods section from a recent PLoS Pathogens Paper using this technique.

In vivo RNAi
C57Bl/6 mice (male, 6–8 weeks) were treated with a single intravenous (i.v.) administration of 5 mg/kg of siRNA formulated in liposomal nanoparticles (Alnylam). Three different modified siRNAs targeting PKC

Alli
 

alligirl88

New member
Nov 16, 2015
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#2

Im still struggling with this experiment. Has anyone out there used RNAi in vivo? We are considering going with the adenoviral or lenti based systems from Clontech - www.clontech.com/products/detail.asp just trying to get any help I can at this point?


AG88