low 260/230 ratio

Hanka

New member
Nov 16, 2015
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#1
Hi, I have a question.



I deal with whole genom amplification. I use for this purpose comercial kit SurePLEX WGA kit. When I measure purity by NANODROP, I have problem with low 260/230 ration. My ratio is about 0,76-0,91. But 260/280 ration is very good (about 1,79-1,83) So where can be a problem ?



Raw material for amplifications are - one cell in 1x PBS buffer, sometimes isolated DNA (diluted in TE buffer).



It can be low 260/230 cause by PBS or TE buffer, or it can cause this problem some components of amplification kit ??



Thanks
 

biomart

New member
Nov 16, 2015
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#2
Hi, after read your questions, I have done some search. Acording to the article from the link: http://www.nanodrop.com/Library/T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf




A low A260/A230 ratio may be the result of:


• Carbohydrate carryover (often a problem with plants).


• Residual phenol from nucleic acid extraction.


• Residual guanidine (often used in column based kits).


• Glycogen used for precipitation.




Hope this help you or you can find explaniations in the article.



Good luck!