Murine heart fixation; perfussion needed??

Nov 16, 2015
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#1
Hello everyone, I'm new in histology techniques. I would like to know if it is better to fix adult hearts embbeding them in PFA4% o/n or fixation through perfussion works better. The hearts will be used for further embbeding in gelatin in order to cryosect.



My mice express both YFP and CFP and I need not only to preserve tissue structure but fluorescence.



Thanks in advance


 

pundir

New member
Nov 16, 2015
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#2
Hi loopingcris,



It is widely accepted that the vascular perfusion of a tissue leads to its optimal fixation for ultrastructural examination. however some research suggest that YFP (Yellow Fluorescent Protein) showed no change between paraformaldehyde-fixed and unfixed samples, however there was a remarkable difference between CFP(Cyan fluorescent protein) samples www.cheng.cam.ac.uk/research/groups/laser/Publications/Clemens/paper_41.pdf