The title says it all really. Im doing some Bisulphite treatment of my gene of interest. The PCR worked fine, ran it on a gel, excised, cleaned, cloned into pGEM, transformed, picked colonies, sent for sequencing all worked, no problem. Repeated on new DNA sample, no bands. Tried a positve control using a clone which I had sequenced and could see had my primer sites in it. No bands. Realiquotted primers. No Bands. Taq works fine for other PCRs no problem. But changed, MG, Taq and cycling conditions. No bands. Re-ordered primers. No bands. Tried denaturing primers and snap cooling them, if secondary structure was a problem. No bands. Used same lanes in PCR machine for other pcrs which worked so not a machin problem. Im pretty stumped.