Thank you for your attention. I have a question about the osmolarity of growth media for embryonic neuronal cell culture.
I saw in literature that the osmolarity of typical neurobasal/B27 growth media is about 235 mOsm. And so was my own measuring. When I measured the osmolarity of my neurobasal/B27 growth media, it was about 225 mOsm.
But, for the external solution, most people recommend about 300 mOsm external solution. Isn’t there any osmolarity shock when the cells are transferred to external solution for recording? I can’t understand why people don’t use 235 mOsm external solution.
Is there anyone who knows the reason?
Sorry for my awful English.