PCR solution

Nov 16, 2015
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#1

DNA quantified well but getting smear in PCR product?

Cry 2 Ab gene zygosity of 2kb PCR product

PCR program is as follows

95d-10min

95d-30sec

60d-1min

72d-1.30min

72d-10min

4d-1min

Reaction

pcr mix-6ml

p1-0.5,p2-0.5,p3-0.5

Tm values

prime1-55 p2-58 p3-67