- Jul 25, 2017
good day, i have problem in separating the peaks from 2 minutes to 6 minutes. There are at least 9 peaks that are combined with each other. The condition of hplc are as follows :
Mobile phase : (A) Acetonitrile - 41% (b) 0.5% formic acid (59%)
Flow : 1 min/ml ; Injection : 20 microlit; Temperature : 30 degrees
Retention time : 40 mins
Solvent extracts : methanol
Column : C18 (250mm x 4.0mm I.D., 5 micronmeter diameter)
I have run the standard and it came out nicely.