Phosphate Buffered Saline (PBS)

Tony Rook

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Nov 16, 2015
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Please find the following media preparation method for:

Phosphate Buffered Saline (PBS)


10X PBS (0.1M PBS, pH 7.2):

Na2HPO4 (anhydrous) --------------------- 10.9 g

NaH2PO4 (anhydrous) ---------------------- 3.2 g

NaCl ------------------------------------------- 90 g

Distilled water ----------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.2

Store this solution at room temperature. Dilute 1:10 with
distilled water before use and adjust pH if necessary.



20X PBS (0.2M PBS, pH 7.2):

Na2HPO4 (anhydrous) ---------------------- 21.8 g

NaH2PO4 (anhydrous) ----------------------- 6.4 g

NaCl ------------------------------------------- 180 g

Distilled water ------------------------------ 1000 ml

Mix to dissolve and adjust pH to 7.2

Store this solution at room temperature. Dilute 1:20 with
distilled water before use and adjust pH if necessary.



10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):

Na2HPO4 (anhydrous) -------------------- 10.9 g

NaH2PO4 (anhydrous) --------------------- 3.2 g

NaCl ------------------------------------------- 90 g

Distilled water ----------------------------- 1000 ml

Mix to dissolve and adjust pH to 7.2 and then add 5 ml of
Tween 20

Store this solution at room temperature. Dilute 1:10 with
distilled water before use and adjust pH if necessary.



20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):

Na2HPO4 (anhydrous) ---------------------- 21.8 g

NaH2PO4 (anhydrous) ----------------------- 6.4 g

NaCl ------------------------------------------ 180 g

Distilled water ------------------------------ 1000 ml

Mix to dissolve and adjust pH to 7.2 and then add 5 ml of
Tween 20

Store this solution at room temperature. Dilute 1:20 with
distilled water before use and adjust pH if necessary.



Note: PBS buffer is used for routine immunohistochemiscal staining. PBS is often used for diluting secondary antibodies or streptavidin-HRP conjugate. PBS-Tween 20 is often used for washing steps.

Link Reference:
http://www.ihcworld.com/_protocols/washing_buffers/pbs.htm