Primers

biomart

New member
Nov 16, 2015
12
0
0
2
#1

Hello


I have some primers and all the primers have the concentration 10 nmoles. I also have the molecular weight of each primers . How can I resuspendthis primers?


Thanks!
 
Nov 16, 2015
0
0
0
2
#2
You can dissolve the primers with 100 ul of 10 mMTrisHCl, pH 8 or TE. This will give you a stock solution of 100 pmoles/ul. For PCR use, you can further dilute the stock 1:10 to give a 10 pmoles/ul working solution. Use 1 ul of the working solution for a volume of 50 ul PCR mix.



Hope this can help you!

Good luck!


 

Pippuri

New member
Nov 16, 2015
2
0
0
2
#3
one additional side note to what creativeproteomics has touched on-



I preferred to prepare the working solution of primers with ddH2O. Since the DNA polymerase reaction is Mg-dependent, by doing the 1/10 dilution with ddH2O can ensure the amount of EDTA in the final PCR reaction become insignificant.



Good luck!