Hi everyone. I have a problem with nested PCR. I keep getting my first PCR product in agarose gel that shows result of my second PCR. The band of first PCR product is faint when i did second PCR after gel purification. However, using the first PCR reaction directly for second PCR gave a strong band on the first PCR product. Although my second PCR product is fine,i want to get rid of the first PCR product. Any idea of how to do that?