Safety cabinet/ laminer flow for transformed bacteria?

kashif

New member
Nov 16, 2015
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#1
I wanted to know what type of safety cabinet/laminer flow I need to use for working with transformed bacteria (for example while growing the bacteria and then isolating of plasmid etc). Whether it should be for user protection or/and product protection. Thanks
 

Tony Rook

New member
Nov 16, 2015
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#2
Please refer to the following link -

Intro to Biological Safety Cabinets

In general - it sounds like you need a Biosafety Cabinet Class II Type A2. This is the most common microbiological safety cabinet and will protect both the user and the product.

To summarize the referenced link above here is some pertinent information regarding selection of biological safety cabinets...

How Biological Safety Cabinets are Classified

Classification Biosafety Level Application
Class I 1,2,3 low to moderate risk biological agents
Class II 1,2,3 low to moderate risk biological agents
Class III 4 high risk biological agents


Here is the air flow diagrams some of the common Class II biosafety cabinets:


Class II, Type A2 Biological Safety Cabinet Design, Baker SterilGARD® III Advance°



Baker Class II, Type B1 Cabinet Design, Baker NCB-C®



Class II, Type B2 Cabinet Design, SterilChemGARD III




References & Credits

All images credited to - Intro to Biological Safety Cabinets


Other links of interest

Labconco's Personnel and Product Protection: A Guide To Biosafety Enclosures
 

Shubhangi

New member
Nov 16, 2015
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#3

Hi


Most of the transformed bacteria are handled at biosafety level ( containment) 2.Its not only to protect user and product but protection of environment is also includedas they generally carry some or other antibiotic resistance gene plus some foreign gene. Depending on the type of gene transfected it is further categorized into higher level or sublevels. But level 2 is minimum.


Person handling them should have minimum training in handling, discard etc.
 
Nov 16, 2015
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#4

The biological safety committee of your organisation should be able to advise you on what biosafety level you should be working at when you submit a risk assessment of the proposed research.
 
Nov 16, 2015
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#7

Recently we have developed a new model of Biological Safety Cabinet(BSC-700II), It is very suitable to resist H1N1 flu,small volume, light weight, easy to delivery by air