smear on the gel

May 20, 2016
1
0
0
2
#1

Dear friends,

What is the reason of smear on the agarose gel?

I ran the PCR products on the agarose gel and saw all the samples have smears.

I use a negative control ( without DNA ) in PCR reaction. there wasn't smear for negative control. Is it possible that the reason of smear is degraded DNA?
 

ARGERINE-1

Administrator
Staff member
Dec 18, 2015
0
0
0
2
#2

If it is in the lower region of the gel it means RNA contamnation if thoughout, it means sample degradation