Theta burst stimulation protocol question (Clampex)

mousy49

New member
Nov 16, 2015
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Hello!

We do extracellular field recording in mouse hippocampi and I have a question about inducing LTP via Theta Burst Stimulation. All my work is usually done with 2 trains of 100Hz (High Frequency Stimulation) but I'm doing an experiment with Theta Burst Stimulation.

I think someone messed up the Clampex program because if I'm right, the slice gets stimulated with 4 pulses at 200Hz instead of 100Hz. Can someone look at this to tell me if it's correct? I have done a lot of ephys on this protocol already and would hate to redo it because it isn't stimulating voltage-gated Calcium channels.

Thanks!

I highlighted things that may be of interest.

Clampex protocol:



Created by: Clampex 10.2.0.18

Acquisition mode: episodic stimulation.

Axon binary file (integer)

40000 samples in this file.

Comment: n/a



Experiment type: other.



Input signal: IN 0 (#0). Telegraphs disabled.

Input signal: IN 1 (#1). Telegraphs disabled.

Input signal: IN 2 (#2). Telegraphs disabled.

Input signal: IN 3 (#3). Telegraphs disabled.

Trial length: 6 runs; 1 sweep of 1000.0 ms (10000 samples per channel).

10.000 s between run starts.

Minimum time between sweep starts.

First sampling rate: 10.00 kHz (interval 100.00 µs).

Second sampling rate: 5.00 kHz (interval 200.00 µs).

Input signals: IN 0 (#0), IN 1 (#1), IN 2 (#2)

and IN 3 (#3).



Signal Cmd 0 (#0) holding level: 0.00 mV.

Signal Cmd 1 (#1) holding level: 0.00 mV.

Signal Cmd 2 (#2) holding level: 0.00 mV.

Signal Cmd 3 (#3) holding level: 0.00 mV.



Analog Output #0

Waveform:

EPOCH A B C D E F G H I J

Type Off Train Step Train Step Train Step Train Step Train

Sample rate Fast Fast Fast Fast Fast Fast Fast Fast Fast Fast

First level (mV) 0 5.0 0 5.0 0 5.0 0 5.0 0 5.0

Delta level (mV) 0 0 0 0 0 0 0 0 0 0

First duration (samples) 0 200 2000 200 2000 200 2000 200 2000 200

Delta duration (samples) 0 0 0 0 0 0 0 0 0 0

First duration (ms) 0.0 20.0 200.0 20.0 200.0 20.0 200.0 20.0 200.0 20.0

Delta duration (ms) 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0

Train Period (samples) 0 50 0 50 0 50 0 50 0 50

Pulse Width (samples) 0 1 0 1 0 1 0 1 0 1

Train Rate (Hz) 0.00 200.00 0.00 200.00 0.00 200.00 0.00 200.00 0.00 200.00

Pulse Width (ms) 0.0 0.1 0.0 0.1 0.0 0.1 0.0 0.1 0.0 0.1

Intersweep holding: same as for signal Cmd 0 (#0).



Digitial Out pattern: disabled.

Pre-sweep train: disabled.

Leak subtraction: disabled.

User list: disabled.