Good day everyone. I have a question which I hope someone could help me solve. I did a PCR, it worked great. I can see the bands on the argarose that is no problem and the amount of DNA is also what I would like to have. My problem lies with the extraction from the gel. I simply do not get the amount of DNA I would like that is if I could believe the Photometer. If I do a ligation my plasmid also seems not to work as my cells do not grow. This gives me a suspicion that the problem lies with the gel extraction.
I am currently in China and the kit I am using is from BioDev it is the B Type: Mini-DNA Rapid Purification Kit. Does anyone has previous experience with this kit or could tell me what I could be doing wrong?
Any help or suggestions would be appreciated.