Sealing films for Real-Time PCR

2 posts / 0 new
Last post
pharmacogenomics's picture
Sealing films for Real-Time PCR

Please indicate which brands you prefer for sealing your real time PCR plates and which problems do you have while using sealing films. Thank you!

Tony Rook
Tony Rook's picture
I am currently running a Bio

I am currently running a Bio-Rad MiniOpticon 48-well real time PCR thermocycler. I have recently been sealing my plates with flat sealing caps, but have realized lately that I have condensation build-up at the end of my cycles. I originally chose to use sealing caps because I was unable to find a sealing tape that was made for 48-well plates. To use the Bio-Rad brand sealing tape, I need to manually cut the tape either before or after sealing the plate. From discussion on this board and within my lab, it seems that sealing tape is a better option than sealing caps for real time PCR applications.