What an incredible forum! Thanks to the admins for forming it.
I am very new to electrophysiology and patch clamping and struggling to understand some of the fundamentals. My question pertains to excitatory postsynaptic currents (EPSCs).
1.) What is the command voltage used for measuing EPSCs during voltage clamping? Is it the resting membrane potential?
2.) During voltage clamping, an influx of cations into the cell will raise the membrane potential and will thus require an influx of negative charge from the current electrode to offset the rise in membrane potential. How does this occur? Does the current electrode supply electrons to the cell?
3.) On a similar note, an influx of anions will lower the membrane potential and will require an influx of positive charge from the current electrode to offset the fall in membrane potential. How does this occur? Does the current electrode remove electrons from the cell?
I trully appreciate all the help that anyone gives me.
Thanks again for this most awesome website/forums.