I'm trying to detect antidromic spike from hippocampal CA1 neuron of acutely prepared brain slice. According to many references, I can get antidromic spike by stimulaing alveus. But I tryed it many times I cannot detect it most of time. Very rarely I could see antidromic spike.
I also tryed getting antidromic spike by putting glass stimulating electrode right next to the patched neuron. But the result was just similar. Even though I can see the antidromic spike rarely, most of time I failed to get it.
Would anyone please let me know any tip for detecting antidromic spike? I have to detect it at single cell level, field recording is not helpful.
Thank you very much.