Hi, dear all,
I just begin my electrophysiological experiment. I try to record fEPSP in hippocampal CA1 after pair-pulse
stimulation to induce LTD.
My problem is that when pair-pulse stimulation(1Hz, 1200 trains) was given, the artifact keeps
changing during this 20mins. This does not always happened. Sometimes LTD can be induced with this
protocol. But everytime, when artifact changing happens, LTD can not be induced successfully. Baseline or after
pair-pulse recordings are quite normal (artifact keeps unchanged). I don't know whether this is because
unstale supply of electric current or maybe there is very trivial movement that can't be detected by bare
eyes(motorized manipulators are used). I changed new bipolar electrodes (FHC Stainless steel electrode). But
it doesn't help.
Anyone has experience in handling pair-pulse stimulation, give me some suggestions to help my
project move on.
Thank you very much!!