Calculate the Liquid junction potential

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Mau
Mau's picture
Calculate the Liquid junction potential

 Dear college,
I do whole cell configuration to record mEPSC in brain slices.
I don't know how to calculate the liquid junction potential. Sould I correct the holding potential if I record the mEPSC?

The below is my ACSF and ICS.

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ACSF
mM

NaCl
119

KCl
2.5

NaH2PO4•0H2O2
1

NaHCO3
26.2

Glucose
11

MgCl2.7H2O
1.3

CaCl2.2H2O
2.5

ICS
mM

CsMeSO4 
135

NaCl
8

HEPES
10

EGTA
0.3

QX-314
5

Mg-ATP
4

Na-GTP
0.3

Spermine
0.1

Thank you,

Best regards.