I am doing acute thalamic slices and am trying to patch cells. I am able to get a gigaohm seal easily, but then am not able to break into the cells to go intracellular. Applying brief pulses of suction or even zapping using the Zap funtion of the MultiClamp 700B is not helping me. I am trying to think of everything that i can be doing wrongm but cant think of anything.
Did anyone have the same problem? If yes, can u suggest me something that I can do (as in change the protocol or solutions a little) to break into the cells?
Cannot break into the cells