I will record the hERG current from 293 cell line (from invitrogen)
The question is I find the cell surface is dirty with much granule on them and the surface seem not smooth and clean .
Can anybody give some suggestion?
please check for mycoplasma contamination
another check the passage number , higher passage increases roughness of surface
if very less particle are there it might be debris.
please check the frequency of alteration of media pH