cel lsurface is dirty

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zhangxu04's picture
cel lsurface is dirty

I will record the hERG current from 293 cell line (from invitrogen)

The question is I find the cell surface is dirty with much granule on them and the surface seem not smooth and clean .

Can anybody give some suggestion?

Abhishek Singh
Abhishek Singh's picture
please check for mycoplasma

please check for mycoplasma contamination
another check the passage number , higher passage increases roughness of surface
if very less particle are there it might be debris.
please check the frequency of alteration of media pH