Channel Open Probability

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Hss's picture
Channel Open Probability

I had been doing wholle cell patch clamp and now I´m planing to do some recordings to see channel open probability for nicotine for the first time, so it will be nice it someone told me how to do it. For example, after doing the patch, should I clamp the cell?, and things like that   (in whole cell I clamp to -70mV).