I have been trying to patch clamp adult dorsal root ganglion neurons for a while now but can't form a gigaseal. My pipette resistance varies between 2-4 Mohms and I very lightly touch the cells before applying suction. The resistance increases upto 20-100 Mohms but I don't get a gigaohm seal. Would really appreciate if somebody could give me tips on how to patch clamp these cells successfully. My extracellular and intracellular solutions are fairly standard (i.e. high Na outside and high K inside). This is the first time I am trying to patch clamp neurons. Could I be doing something wrong?
Thanks in advance.
Difficulty in patch clamping neurons