I've never been on this site its te first time i've discovered it and it seems pretty useful as I've been reading different threads and posts on here.
I've been using the HT ionworks machine and have been experiencing problems with it
No matter how careful we are with cells were still getting large seal failures ...
1) can it be due to low channel expression or vaccume under the patchplate?
2) can the passage number of cells have and affect on cell size and therefore leading to large number of seal failures?
3) anyone ever looked into how the cell size can affect the seals of cells on a patchplate?
4) the antibiotic used for selecting the whole cells that express the channels ... is there any test to determine if the cells have developed a resistance to the agent and therefore no longer selective?
sorry for asking too many questions ... is anyone using the same machine? its been a pain getting this thing to optomise
we get odd days when the assay has worked really well and then a few odd weeks where its no longer functioning anymore ...