hi I have got some questions when recording hERG tail current.
protocol: -90mv for 300ms, +40mv for 2s , -10mv for 2s and -90mv for 300ms
1: I get largest tail current amplitude at -10mv, but it is reported that the largest tail curent amplitude should be induced at about -50 or -40mv
2: sometimes I can not record the tail current while the stable current (at +40mv) is good.
can soembody give me suggestions?