I am trying to do some field recording in hippocampal slices but I have a problem.
I think that my slices for some reason are not healthy, because when I put the recording electrode in the stratum radiatum I first see a good fEPSP but it decreases until disappearing in a few minutes. I am using a vt1200 leica vibratome, and after decapitating the animal I put the brain in a becker with chilled modified acsf with sucrose. After doing the slices of 350uM thickness I put the slices in normal acsf at 30 degrees and I wait two hours of recovery before using it for the recordings.
Any suggestions for my problem???
Hippocampal slices for field recordings