I'm trying to see a specific current generated from one type of channel.
To do that I'm considering subtracting most other currents from the whole currents by blocking the specific channel. What I'm wondering is whether I can do those subtraction work in pClamp software. Otherwise, is there any good way to do that?
Thanks for reading.
Yes as long as you sample at the same rate when you acquire the data you can subtract two currents from one another in Clampfit.
Look for the Add / Subtract traces function
You can isolate individual sweeps from different files, compile them in a new file and subtract them. You can even write a macro to do it for you
Thank you very much~! Your reply was really helpful.