Leaking submersion chamber / constant detachment of the glass bottom

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TMBGJO
TMBGJO's picture
Leaking submersion chamber / constant detachment of the glass bottom

 Hi everybody,

I'm having a rather annoying problem, namely we have got a new patch-clamp set-up in the lab and when I wanted to record ljps yesterday I observed that the coverglass constantly detached from my submersion chamber.

On the previoius set-up we used to seal the chamber by using one layer of parafilm and then "soldered" the glass to the parafilm. It worked fine. Unfortunately I have no chance to go back to using the previous chamber.

I would appreciate if you could throw some advice my way.

Cheers!

The Frog Blog
The Frog Blog's picture
 A picture of your chamber or

 A picture of your chamber or the part numbers of with the manufacturer's details might help get some more specific answers to your request.

TMBGJO
TMBGJO's picture
@The Frog Blog

@The Frog Blog
Indeed, that is a good point. The chamber I use the plastic "Slice Recording Chamber" from Scientifica.

TMBGJO
TMBGJO's picture
 Turned out I had to use

 Turned out I had to use silicone in the end. Now it works.

zazation
zazation's picture
If you will have this problem

If you will have this problem again you can do another one thing. 
Just put a piece of parafilm of the same shape of the glass, between the chamber and the glass itself.
Then melt the parafilm, just warming the glass with an iron solderer.  When you see that the parafilm becomes transparent it means that it is melted but  remember that you should not melt the parafilm which is in the centre of the chamber cause you have to remove it with a blade, otherwise the light of the microscope will not reach the tissue properly.
I can assure you that if you don't break the glass you will never have to change the glass, unless it becomes dirty.
I tried to use the silicone but I noticed that it released some dirt on my slices.

good luck!