Making up Internal Solution

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The Happy Clamper
The Happy Clamper's picture
Making up Internal Solution


I am concerned about making up my internal solution, aliquoting into 500ul then freezing. Is this the usual method people use?

Philly's picture
 yes, except that my aliquots

 yes, except that my aliquots are 1 ml

malumno's picture
Why would you not recommend

Why would you not recommend storing the solution in the fridge?

g a
g a's picture


If the internal solution conatins BSA or other protein, it will be degraded at RT or refrigerator as well as may even lead to microbial growth. So it is not stored in fridge but freezed.

dansusername's picture
Freezing is necessary if you

Freezing is necessary if you are adding ATP and GTP to your aliquots to prevent ATP/GTP degradation.