We're trying to set up a rig for patch clamping. We've got the Axopatch 200B amplifier, and Clampex 10.2. The signals and input/output channels seem to be correctly configured. For example, if we put an electrode in the bath, and run seal test from the amplifier, we get square voltage pulses, and corresponding ~square current responses in the oscilloscope window in Clampex.
I would like to use the "membrane test" tool in Clampex to monitor the resistance when forming a seal. We configured the input and output signals in the membrane test configuration window, but then when I try to click "membrane test" in the Tools menu, nothing happens... From the Clampex manual, I believe there's supposed to be a dialog box, with buttons for the stages called "bath", "seal", and "cell"...
Any help would be greatly appreciated!!
Membrane Test tool in Clampex