Noise while using suction in perfusion chamber

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sakthikumar
sakthikumar's picture
Noise while using suction in perfusion chamber

 Hi

I'm currently working on DRG whole cell recordings in patch clamp and I am trying to use continuous perfusion mode.Everything is fine till giga seal formation even after switching on the perfusion ;but when I use the vaccum pump to remove the buffer from the chamber i am getting strange noise.I have checked all the ground and everything is fine , my only problem is when I use the vaccum pump to remove the buffer through suction.Could anybody give some suggestions???

Thank you

Sakthikumar

sharmakaushikp
sharmakaushikp's picture
Try using a bubble trap just

Try using a bubble trap just after your suction tubing leaves the rig. This will cause the flow of solution in your suction tubing to become discontinuous, and so helps in eliminating the noise caused due to the vacuum pump to a great extent. It works extremely well in my case, just that you have to keep on replacing the trap with a new piece regularly (in 3-4 weeks time approx).

Goodluck.

The FFM
The FFM's picture
Is the tip of your suction

Is the tip of your suction line metal?

make sure you have a ground wire connected right to that metal tip (ie. not just to the clamp holding it in place) and run the wire directly to your ground plate.  Do not loop it in with any of your other ground wires.

engstar
engstar's picture
Where do you position your

Where do you position your bottle that collects the removed solution? It should NOT be placed out of the shielding cage.