P/4 protocol

1 post / 0 new
joy12's picture
P/4 protocol

Could anyone tell me what exactly is a P/4 protocol and how is it implemented? How does it help in leak and capacitive current subtraction?
For my experiments, I want to measure the gating current (to normalize for differences in channel expression) and tail current (Ba2+) in HEK cells expressing L-type Ca2+ channels. Also, is there a specific protocol to measure the tail current?

I got these details from:
Hulme, J. T., Yarov-Yarovoy, V., Lin, T. W.-C., Scheuer, T. and Catterall, W. A. (2006), Autoinhibitory control of the CaV1.2 channel by its proteolytically processed distal C-terminal domain. The Journal of Physiology, 576: 87–102. doi: 10.1113/jphysiol.2006.111799

Thank you.