Pair-recordings in neuronal cell culture

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Rene
Rene's picture
Pair-recordings in neuronal cell culture

I am working with Double-EPC-9 (HEKA) and try to make pair-recordings (whole-cell patch clamp) in neuronal cell cultures. Both probes are connected from GND to the same bath electrode. The recorded currents from one probe are always highly noisy, but if I disconnect the wire between GND1 of Probe1 to the bath electrode or between GND2 of Probe 2 and the bath electrode, then the currents from the remaining connected Probe are o.k.. The same happens also, if each Probe has an own electrode. For me it seems to be, that between both Probes is feedback? Who has ideas to prevent the feedback induced noise?
Thanks for comments. Rene

Guy Sovak
Guy Sovak's picture
Hi.

Hi.
I will try getting a solution for you
I have heard about feedback between two electrodes, never heard any way to solve it, but I will try again to find some.

Guy Sovak
Guy Sovak's picture
Hi

Hi
I asked arround and people told me that you might try to play with the detection threshold and adjusted between 6 and 15 pA, depending on the noise of the recording.

Rene
Rene's picture
I discussed this problem now

I discussed this problem now with HEKA directly,and this guys assume, there should be an errors in the EPC9/2. So, I send my EPC9/2 to HEKA and I hope they will find something (I my case the noise was >500 pA). Sorry for my late response.