I am doing patch clamp in HEK cells. Very often I have problem with not having gigaseal at all. In case I succeed with giga seal the patches are not stable at all. I am using the foloowing solutions
extracellular solution in mM: NaCl 135, KCl 5, HEPES 10, Glucose 10, MgCl2 1, Cacl2 1
intracellular solution in mM: KCl 140, NaCl 4, MgATP 2, HEPES 10, EGTA 5, Cacl2 2,2
I will appreciate if you have suggestions and comments.
Thanks a lot in advance.