patch clamp and cell death

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egggead
egggead's picture
patch clamp and cell death

 I am doing some action potential recording for human embryonic stem cell derived cardiomyocytes, my problem is that right after I got the G-seal, my cell dead and I could not get any recording, even though it beat second before I patched the cell. We can get action potential recording from 3-5 cell cluster, also we can record the currnt from sigle cardiomycoytes. Do you think our buffer system kills the cell?  

Appreciate your input!

BigPharmBoy
BigPharmBoy's picture
I guess I would measure the

I guess I would measure the access resistance after going into the whole cell configuration. If access resistance is too low, then you have a leaky seal, which would explain the lack of action potentials.

But I think this issue has been addressed on these forums in the past. Here is the link:

http://www.scientistsolutions.com/t9329-cardiomyocyte+patch+clamping.html

I hope that helps.

chrismathes
chrismathes's picture
You should try perforated

You should try perforated patch clamp!
 

egggead
egggead's picture
 Thank you very much for your

 Thank you very much for your help!

I will try.