Perforate patch clamp with nystatin

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Olf23
Olf23's picture
Perforate patch clamp with nystatin

Hi,

I'm doing perforate patch clamp with nystatin. I have a problem with my pipet resistance (R), the resitance in the bath should be around 15-25, but I have 45-60, I redid my pipet solution, sonicate nystatin in DMSO, and my pipet solution + nystatin in DMSO. I cheked the puller filament ! but I still have a higher pipet resistance. it is a nightmare !! Do you have please  any suggestions!!

Thank you in advance for yout ansewers....

Regards.

Olf23.

chrismathes
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Have you considered trying

Have you considered trying amphotericin B or Escin?  If you're stuck with nystatin be sure to use Pluronic acid to help dissolve the nystatin.

Olf23
Olf23's picture
 Hi Chrismathes,

 Hi Chrismathes,

Thank you for your answer... I will  try Amphotercin B...

Olf23

Olf23
Olf23's picture
 Hi chrismathes, 

 Hi chrismathes, 

it was the puller program, I changed the program the resistance is OK. Again thank you for your advice.

Best, 

Olf23