Procedure of Whole cell patch clamp

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RedBull
RedBull's picture
Procedure of Whole cell patch clamp

I had found a link on teaching me how to control the EPC 9 amplifier:

http://www.heka.com/support/Tutorials/StartPulse.pdf

Can anyone suggest to me if the website is useful for me to get familar with the patch clamp procedure?

Thanks!

Fraser Moss
Fraser Moss's picture
Whether you use Axon, Heka,

Whether you use Axon, Heka, or Dagan equipment you should always run through the manufacturer's digagnostic procedures with the model cell to familiarize youself with the set up and the patching procedure. If you are ineed a beginner and the manual you linked to in your post is the correct one for the amplifier on your rig, then definitely run through all the basic procedures from cover to cover,no matter how trivial they may seem.

Of course working with the model cell is not like getting a seal and working with a real cell. But you should be confident in "driving" your rig before you even get to patching a cell.

You may also want to read the Axon guide

http://www.moleculardevices.com/pages/instruments/axon_guide.html

I can also reccomend the following book for beginners

Patch Clamping : An Introductory Guide to Patch Clamp Electrophysiology

by Areles Molleman

# Publisher: John Wiley & Sons (December 6, 2002)
# Language: English
# ISBN: 047148685X

and of course there is always the classic

Ion Channels of Excitable Membranes (3rd Edition)
by Bertil Hille.

# Publisher: Sinauer Associates; 3rd edition (July 1, 2001)
# Language: English
# ISBN: 0878933212

RedBull
RedBull's picture
Really thanks to your help.

Really thanks to your help.

It does give me assistance.

Regarding the Axon Guide, I had read all the 300 pages TWICE.

For the suggested books, I will definitely try them.

Really thanks to you!!

littlebush
littlebush's picture
Ion Channels of Excitable

Ion Channels of Excitable Membranes (3rd Edition)

I have dreamed for having on such book for a very long time ,however,very unfortunately,being a very poor Ph.D student who have only 30 dollars a month for living,it is impossible for me to afford that price.Who can tell me if there is possibility for my getting a free one?Thanks for your suggestion and best regards.Do not play jokes on me and I am really very serious.

RedBull
RedBull's picture
littlebush wrote:Ion Channels

littlebush wrote:

Ion Channels of Excitable Membranes (3rd Edition)

I have dreamed for having on such book for a very long time ,however,very unfortunately,being a very poor Ph.D student who have only 30 dollars a month for living,it is impossible for me to afford that price.Who can tell me if there is possibility for my getting a free one?Thanks for your suggestion and best regards.Do not play jokes on me and I am really very serious.

I wanna ask,

which country are you from?

lost_marbles
lost_marbles's picture
China?

China?

littlebush
littlebush's picture
I have read both Heka and

I have read both Heka and Axon amplifier manuals .I have to say if you want to be familiar with the whole cell or single cell recording procedures ,please read the Axon's munuals instend of that of Heka regardless which amplifier at your hand.Very detailed and good description on how to perform the patch manipulation are listed step by step in the Axon's manual such as the 200B ,which you can download freely from the Molecular Device company's website.I want to add in the end for patch clamping,always show enough patience and persistance .Whatever fructration you may encounter ,please believer in that such indevour will certaily pay back and the lab is revarding. If you have any problems ,please do not forget logging onto this website for help.I think everyone with more experience will lend his hand.

Hope that will help.