QX-314 in the internal solution for patch clamp evoked potentials

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zazation
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QX-314 in the internal solution for patch clamp evoked potentials

Hi,
I have a very simple question.
If I want to record evoked currents (paired Pulses, eEPSC etc) in whole-cell patch clamp configuration, do I have to put QX-314 in the internal solution?

c.hill
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From what I understand, QX

From what I understand, QX-314 blocks voltage-gated Na channels by binding to the intracellular portion. However, QX-314 is not inherently membrane permeable and so enters neurons via TRPV1 channels. The decision to apply it internally or externally will depend on the cells you're patching.

Hope that helps,
c.hill

zazation
zazation's picture
I didn't know about TRPV1

I didn't know about TRPV1 permebility to QX. Thank you for the info, but my concern was about using it or not to record evoked currents! Does it make some difference for example if I want to record a Paired Pluses evoked currents with or without QX?

c.hill
c.hill's picture
QX-314 seems to block all

QX-314 seems to block all sorts of voltage-dependent currents, so it will probably have an effect on the shape of evoked EPSCs.

I found this:
http://archimede.bibl.ulaval.ca/archimede/fichiers/22126/ch06.html

The third section of the Results is titled 'Effects of QX-314 on evoked EPSPs', so you should probably take a look and see what you think.