hello all,
i have been trying to get sodium current recordings from rat neonatal myocytes via whole cell patch and have been running into considerable issues. I can get cell attached patches regularly and sometimes can go whole cell but often the cells abruptly die. my solutions for these recordings are as follows (all in mM).
Bath: Pipette
25 NaCl, 10 NaF
118.2 NMDG 110 CsF
4 KCl 20 CsCl
1 MgCl2 10 EGTA
1.8 CaCl2 10 HEPES
10 HEPES pH to 7.4 w/ CsOH
10 Glucose
-pH to 7.4 w/ HCl
does anybody have experience with recordings from this cell type? and if so i would very much like to hear from you as well as well as anybody who can suggest some solutions as this has been going on for several weeks now.