recording in WinWCP

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veronika's picture
recording in WinWCP


I had to start to use WinWCP recording software for whole cell patch clamp recordings. It seems to be good and I managed to synchronise with the amplifier (Multiclamp 700B) and with teh interface (CED 1401). It can see the gain changes or the switch between VC and IC. But somehow the pipette resistance values are not good. I can check teh resistance with teh amplifier (5-6 MOhm) and teh software shows values between -2 and +1 MOhm, also it does not show teh increase of the pipette resistance during sealing. The continous readout of the resistance during sealing is crutial to get a gigaseal. Anyone has any experience with WinWCP recording software? or any idea what can be teh problem?