voltafe gated K channel

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Postgrad's picture
voltafe gated K channel

<br /> I'm transfecting my cells with a cdna for voltage gated potassium channel. The K reversal potential is -83 (From literature) but I'm getting a negative values as seen in the graph below when going in 20mv steps from -60 to +80 and I'm holding the membrane potential at -80. <br /> <br /> The graph below represent the outward current at the given voltage.<br /> <br /> Any ideas what is going on?<br /> <br />

fatchains's picture
A few questions:

A few questions:
What is the jxn potential?
Do you have a good seal?
What is the Rm at rest (Rin)?
What type of headstage and gain setting are you using?