Voltage Sensitive Dyes

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Hss
Hss's picture
Voltage Sensitive Dyes

 
Hi 
We would like to begin to work with voltage sensitive dyes, so we are begginers in this field. So far we made Ca++ imaging with Fura-2 and electrophysiology (patch clamp whole cell). Now we would like to see the changes in the membrane potential with dyes, without patching, just depolarizing the cell by local (pressure) application of agonist; we need to know which kind of filter we need and which dyes can be better  and so. All suggestions are welcome.
Thanks. 
 
 

heehawmcduff
heehawmcduff's picture
You can use Bis-oxononol

You can use Bis-oxononol based dyes to look at changes in membrane potential as these are a family of voltage sensitive dyes.
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Probes-for-Membrane-Potential/Slow-Response-Probes.html

hilltrekker
hilltrekker's picture
Millipore filter . Details

Millipore filter . Details about dyes can be found on this webpage
http://info.med.yale.edu/cmphysiol/cohen/whichdye.html
I hope it covers many details in a tabulated form, thus more informative.

Hss
Hss's picture
 ...do you know if we can use

 ...do you know if we can use the same system (software and filters) from calcium imaging for the voltage sensitive dyes?
Thanks.