I'm trying to do cell attached patch, and I want to use sylgard to reduce capacitance.
I read about using sylgard many times here and there, but still I don't know exactly how I can use it.
Do they actually mean that I have to paint sylgard with something like brush? close to the tip of the pipette? under the microscope?
Or can I just immerse it to sylgard solution?
Anyone who can let me know about it in detail, please?