first: when pipette touch cell, I can see a increase of the resistance, but at the same time the baseline whobble greatly. I orignally think I press the cell too much, but even I slightly move the pipette onto the cell, the baseline also wobble greatly. can you teach me how this happen ?
second: it need too long to establish the G ohm seal seal, about 5 minutes or even more. I think this is not correct.I do not know why
third: mostly, this is the most distriuting point. I can not eatablish G ohm seal, always about 40-50M ohm, then seal stop increasing. I further sucked, but not increase of seal, still about 40-50M ohm. at this moment, I find there is much of cell membrane-likes in the pipiette. when I lift up the pipette, there left a relative long and thin "tail" on the brim of cell (just like "Q" as you imaging). more strongly suck, more lone of this type of tail, but the seal can not increase.
I have check my system, not air leak.
so please help me.