Assay Development

1 post / 0 new
ldemers
ldemers's picture
Assay Development

We have access to a purified tumor associated peptide along with polyclonal antibody to develop an ELISA. We would like a detailed protocol to develop an ELISA quickly using a microtiter plate format. Would like specific recommendations regarding how to coat the plates with antibody to insure that it sticks and suggestions for developing an ELISA as opposed to an IRMA.