hi, I've examed several conditions (temperature, time, concentration of inducer) to induce scFvs. However, it seems to express low-level (2mg/L) in experiments. The procedure was based on Barbas manual. Should we try other details?
bateria strin: HB2151
temperature:30 and 37 (o/n)
induction condition: IPTG 0.1mM-1mM (final) at A600=0.6-0.7